Injection molded nanofluidic chips: fabrication method and functional tests using single-molecule DNA experiments.
نویسندگان
چکیده
We demonstrate that fabrication of well-defined nanofluidic systems can be greatly simplified by injection molding of thermoplastic polymers. Chips featuring nanochannel arrays, microchannels and integrated interconnects are produced in a single processing step by injection molding. The resulting open channel structures are subsequently sealed by facile plasma-enhanced thermal bonding of a polymer film. This fast, inexpensive and industry-compatible method thus provides a single-use all-polymer platform for nanofluidic lab-on-a-chip applications. Its applicability for nanofluidics is demonstrated by DNA stretching experiments performed on individual double-stranded DNA molecules confined in the injection molded nanochannels. The obtained results are consistent with measurements performed in costly state-of-the-art silica nanochannels, for both straight and tapered channel geometries.
منابع مشابه
Wafer-scale integration of sacrificial nanofluidic chips for detecting and manipulating single DNA molecules
Wafer-scale fabrication of complex nanofluidic systems with integrated electronics is essential to realizing ubiquitous, compact, reliable, high-sensitivity and low-cost biomolecular sensors. Here we report a scalable fabrication strategy capable of producing nanofluidic chips with complex designs and down to single-digit nanometre dimensions over 200 mm wafer scale. Compatible with semiconduct...
متن کاملHigh throughput fabrication of disposable nanofluidic lab-on-chip devices for single molecule studies.
An easy method is introduced allowing fast polydimethylsiloxane (PDMS) replication of nanofluidic lab-on-chip devices using accurately fabricated molds featuring cross-sections down to 60 nm. A high quality master is obtained through proton beam writing and UV lithography. This master can be used more than 200 times to replicate nanofluidic devices capable of handling single DNA molecules. This...
متن کاملFiber-Based, Injection-Molded Optofluidic Systems: Improvements in Assembly and Applications
We present a method to fabricate polymer optofluidic systems by means of injection molding that allow the insertion of standard optical fibers. The chip fabrication and assembly methods produce large numbers of robust optofluidic systems that can be easily assembled and disposed of, yet allow precise optical alignment and improve delivery of optical power. Using a multi-level chip fabrication p...
متن کاملEfficient prototyping of large-scale pdms and silicon nanofluidic devices using pdms-based phase-shift lithography
In this study, we explore the potential of Poly-DiMethylSiloxane (PDMS)-based phase shift lithography (PPSL) for the fabrication of nanofluidic devices. We establish that this technology, which was already shown to allow for the generation of 100 nm linear or punctual features over cm surfaces with conventional photolithography systems, is readily adequate to produce some of the most popular na...
متن کاملFemtosecond Laser 3D Fabrication in Porous Glass for Micro- and Nanofluidic Applications
The creation of complex three-dimensional (3D) fluidic systems composed of hollow microand nanostructures embedded in transparent substrates has attracted significant attention from both scientific and applied research communities. However, it is by now still a formidable challenge to build 3D microand nanofluidic structures with arbitrary configurations using conventional planar lithographic f...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Lab on a chip
دوره 11 2 شماره
صفحات -
تاریخ انتشار 2011